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avidin biotin peroxidase complex solution  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 16815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectastain+elite+abc+hrp+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Universal)/pm42892892-383-17-25
    Average 96 stars, based on 16815 article reviews
    avidin biotin peroxidase complex solution - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Voluntary physical activity modulates brain ΔFOSB and alters co-activation networks in male and female mice
    Article Snippet: In short, free-floating sections frommale brains were washed in 0.05 M Tris-buffered saline (TBS), endogenous peroxidase activity was inhibited by pretreating for 10 min with 1.5% v/v H202 (Merck; in TBS) before incubation with rabbit anti-FOSB [5G4; #2251, Cell Signaling Technology, RRID: AB_2106903; 1:1,000; this antibody recognizes FOSB, ΔFOSB, variant FOSB (vFOSB), and Δ2ΔFOSB in striatal nuclear extracts, which are all absent in fosB-null mice (Ohnishi et al., 2011)] in supermix (0.25% w/v gelatin, 0.5% v/v Triton X-100, in 1× TBS), pH 7.6, for 1 h at room temperature (RT) and overnight at 4°C. .. Following incubation with a biotinylated goat anti-rabbit IgG (H+L; BA-1,000; Vector Laboratories, RRID: AB_2313606; 1:200 in supermix) for 2 h at RT, signal amplification was achieved using avidin–biotin complex (Vectastain Elite ABC HRP Kit, PK-6100, Vector Laboratories; 1:800 in supermix) for 1.5 h at RT and visualized by nickel-enhanced DAB [0.05% w/v 3.3-diaminobenzidine (Sigma-Aldrich), 0.23% w/v nickel-ammonium sulfate (Merck), and 0.01% w/v H2O2 (Merck) in 0.05 M TB], pH 7.6. .. Sections were then mounted onto SuperFrost Plus slides (Thermo Fisher Scientific), air- October 2026, 13(10).

    Article Title: Assembly of bioinspired multifunctional microspheres for enhanced alveolar bone regeneration
    Article Snippet: .. After deparaffinization and antigen retrieval, tissue sections were incubated with primary antibodies followed by detection using the Vectastain Elite ABC-HRP kit (Vector Laboratories, Burlingame, CA) according to the manufacturer's protocol. ..

    Article Title: Experimental Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus Infection in Alpacas, 2026.
    Article Snippet: We performed immune cell labeling by using a 1:500 dilution of Anti Iba-1, Rabbit (FUJIFILM Wako Chemicals Europe, https:// labchem-wako.fujifilm.com) antibodies against ionized calcium binding adaptor molecule 1 (Iba-1) targeting microglia and macrophages. .. To detect Iba-1 antibodies, we used biotinylated goat anti-rabbit IgG secondary antibody and incubated slides with Vectastain Elite ABC-HRP Kit (both Vector Laboratories, https://vectorlabs.com). .. We also targeted T cells by using a 1:100 dilution of Polyclonal Rabbit Anti-Human CD3 (DAKO Agilent, https://www.

    Article Title: Sexual dimorphism in epidermal stem cell dynamics.
    Article Snippet: .. Tissue sections were incubated overnight with the primary antibody, washed and incubated for 30 min using a Vectastain Elite ABC-HRP kit (peroxidase, rabbit IgG) (Vector Laboratories). ..

    Amplification:

    Article Title: Voluntary physical activity modulates brain ΔFOSB and alters co-activation networks in male and female mice
    Article Snippet: In short, free-floating sections frommale brains were washed in 0.05 M Tris-buffered saline (TBS), endogenous peroxidase activity was inhibited by pretreating for 10 min with 1.5% v/v H202 (Merck; in TBS) before incubation with rabbit anti-FOSB [5G4; #2251, Cell Signaling Technology, RRID: AB_2106903; 1:1,000; this antibody recognizes FOSB, ΔFOSB, variant FOSB (vFOSB), and Δ2ΔFOSB in striatal nuclear extracts, which are all absent in fosB-null mice (Ohnishi et al., 2011)] in supermix (0.25% w/v gelatin, 0.5% v/v Triton X-100, in 1× TBS), pH 7.6, for 1 h at room temperature (RT) and overnight at 4°C. .. Following incubation with a biotinylated goat anti-rabbit IgG (H+L; BA-1,000; Vector Laboratories, RRID: AB_2313606; 1:200 in supermix) for 2 h at RT, signal amplification was achieved using avidin–biotin complex (Vectastain Elite ABC HRP Kit, PK-6100, Vector Laboratories; 1:800 in supermix) for 1.5 h at RT and visualized by nickel-enhanced DAB [0.05% w/v 3.3-diaminobenzidine (Sigma-Aldrich), 0.23% w/v nickel-ammonium sulfate (Merck), and 0.01% w/v H2O2 (Merck) in 0.05 M TB], pH 7.6. .. Sections were then mounted onto SuperFrost Plus slides (Thermo Fisher Scientific), air- October 2026, 13(10).

    Avidin-Biotin Assay:

    Article Title: Voluntary physical activity modulates brain ΔFOSB and alters co-activation networks in male and female mice
    Article Snippet: In short, free-floating sections frommale brains were washed in 0.05 M Tris-buffered saline (TBS), endogenous peroxidase activity was inhibited by pretreating for 10 min with 1.5% v/v H202 (Merck; in TBS) before incubation with rabbit anti-FOSB [5G4; #2251, Cell Signaling Technology, RRID: AB_2106903; 1:1,000; this antibody recognizes FOSB, ΔFOSB, variant FOSB (vFOSB), and Δ2ΔFOSB in striatal nuclear extracts, which are all absent in fosB-null mice (Ohnishi et al., 2011)] in supermix (0.25% w/v gelatin, 0.5% v/v Triton X-100, in 1× TBS), pH 7.6, for 1 h at room temperature (RT) and overnight at 4°C. .. Following incubation with a biotinylated goat anti-rabbit IgG (H+L; BA-1,000; Vector Laboratories, RRID: AB_2313606; 1:200 in supermix) for 2 h at RT, signal amplification was achieved using avidin–biotin complex (Vectastain Elite ABC HRP Kit, PK-6100, Vector Laboratories; 1:800 in supermix) for 1.5 h at RT and visualized by nickel-enhanced DAB [0.05% w/v 3.3-diaminobenzidine (Sigma-Aldrich), 0.23% w/v nickel-ammonium sulfate (Merck), and 0.01% w/v H2O2 (Merck) in 0.05 M TB], pH 7.6. .. Sections were then mounted onto SuperFrost Plus slides (Thermo Fisher Scientific), air- October 2026, 13(10).

    Tissue:

    Article Title: Assembly of bioinspired multifunctional microspheres for enhanced alveolar bone regeneration
    Article Snippet: .. After deparaffinization and antigen retrieval, tissue sections were incubated with primary antibodies followed by detection using the Vectastain Elite ABC-HRP kit (Vector Laboratories, Burlingame, CA) according to the manufacturer's protocol. ..

    Article Title: Sexual dimorphism in epidermal stem cell dynamics.
    Article Snippet: .. Tissue sections were incubated overnight with the primary antibody, washed and incubated for 30 min using a Vectastain Elite ABC-HRP kit (peroxidase, rabbit IgG) (Vector Laboratories). ..

    Staining:

    Article Title: A scalable workflow for sensitive detection and genomic profiling of tumour cells in lymph nodes from FFPE tissue.
    Article Snippet: The slides were allowed to cool in the solution for 20 min at room temperature and were subsequently washed in PBS for 5 min. To inhibit endogenous peroxidase activity, the sections were incubated with BLOXALL blocking solution SP-6000 (Vector Laboratories, Burlingame, CA,USA) for 10 min (or 0.3% hydrogen peroxide for 30 min) followed by washing with PBS for 5 min. .. Sections were subsequently stained using the VECTASTAIN Elite ABC-HRP kit (Vector Laboratories) following the manufacturer’s protocol, performing only the quenching of endogenous peroxidase activity but not the antigen unmasking step. .. Primary antibody staining was performed overnight at 4 C using the following: anti-EpCAM (Cf = 0.75 μg/ml, 1:400, Clone VU1D9, Cat no. 2929S, Cell Signaling Technology, Danvers, MA, USA); or antipanCK (Cf = 2.5 μg/ml, 1:200, Clone C11, Cat no. 628602, Biolegend, San Diego, CA, USA); or the corresponding isotype control (Cf = 2.5 μg/ml, 1:200, Clone MOPC-21, Cat no. 400102, Biolegend).

    Activity Assay:

    Article Title: A scalable workflow for sensitive detection and genomic profiling of tumour cells in lymph nodes from FFPE tissue.
    Article Snippet: The slides were allowed to cool in the solution for 20 min at room temperature and were subsequently washed in PBS for 5 min. To inhibit endogenous peroxidase activity, the sections were incubated with BLOXALL blocking solution SP-6000 (Vector Laboratories, Burlingame, CA,USA) for 10 min (or 0.3% hydrogen peroxide for 30 min) followed by washing with PBS for 5 min. .. Sections were subsequently stained using the VECTASTAIN Elite ABC-HRP kit (Vector Laboratories) following the manufacturer’s protocol, performing only the quenching of endogenous peroxidase activity but not the antigen unmasking step. .. Primary antibody staining was performed overnight at 4 C using the following: anti-EpCAM (Cf = 0.75 μg/ml, 1:400, Clone VU1D9, Cat no. 2929S, Cell Signaling Technology, Danvers, MA, USA); or antipanCK (Cf = 2.5 μg/ml, 1:200, Clone C11, Cat no. 628602, Biolegend, San Diego, CA, USA); or the corresponding isotype control (Cf = 2.5 μg/ml, 1:200, Clone MOPC-21, Cat no. 400102, Biolegend).



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: VECTASTAIN Elite ABC-HRP Kit, Peroxidase , Vector Laboratories , Cat# PK-6100.

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy